Host-parasites relationships among Shistosoma mansoni and Echinostoma liei with their intermediate host Biomphilaria alexandrina using RAPD-PCR

Biomphalaria alexandrina are known to be intermediate hosts for both shistosoma mansoni and Echinostoma liei RAPD-PCR assay offers a new approach to host-parasite relationships . This was performed by investigating the genetic variation and compatibility among S. mansoni ,E. liei and their intermediate host B. alexandrina with special emphasis on variations occurring in snails infected with S.mansoni and /or E.liei. Six primers were screened for DNA analysis and gave total patterns from 28 to 37 reproducible bands for each species . all specimens analyzed by RAPD-PCR gave interpretable electrophoric banding pattern that were polymorphic and compatible in the amplified products of these primers within each species

August 2004

Journal of the Egyptian Society of Parasitology 34(2):577

Souad M Alsaqabi